RedShiftBio Lab Photo

App Notes

App Notes

Assessment of Quantitation Linearity for Bovine Serum Albumin Using MMS vs FTIR

December 1, 2020


This Application Note Includes:

  • BSA was prepared as a series and examined by MMS to measure the concentration linearity of the series.
  • Measures protein secondary structure by combining infrared spectroscopy with microfluidics to enhance sensitivity and accuracy
  • Achieves higher sensitivity across a wider concentration range compared to current FTIR techniques

FTIR Protein Analysis Of MMS

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1). MMS is performed on the AQS³pro system from RedShift BioAnalytics, and it generates measurements over a wide range of complex backgrounds. The AQS³pro operates using a quantum cascade laser in combination with a microfluidic flow cell resulting in a wide dynamic range of 0.1 to >200 mg/mL for structural characterization, and further extended to 0.01 to >200 mg/mL for quantitation measurements.

MMS surpasses FTIR for differentiating higher order structure changes without interference of excipients or loss of linerarity at low or high concentrations.

Please complete the form if you would like to speak to download the app note.

Request a Demo Today! 

RedShiftBio AQS3 Product Detail