Microfluidic Modulation Spectroscopy (MMS) is a novel spectroscopic tool which directly addresses the limitations of current technologies by facilitating direct, label-free analysis of secondary structure over a wide concentration range (0.1 to 200 mg/mL) in complex formulations without the need for buffer exchange or dilution. MMS employs a tunable mid-infrared quantum cascade laser that is 1,000 times brighter than conventional FTIR. In addition, MMS enables the measurement of currently undetectable changes in biomolecule structure. Now with the power of the AQS³pro MMS system, the sample (biomolecule-in-buffer) solution and a matching buffer reference stream are introduced into the transmission cell under continuous flow and then rapidly modulated (1–5 Hz) across the laser beam path. This produces nearly drift free, background compensated, differential absorbance scans of the Amide I band (1700-1600 cm-1) and allows changes in HOS to be easily measured and compared.
Microfluidic Modulation Spectroscopy is a novel tool that can strengthen a bioanalytical tool kit by providing critical insight through all phases of drug development. This technique enhances current toolkits with the ability to monitor 5 key biomolecule characteristics including aggregation, quantitation, structure, stability, and similarity. MMS provides the following benefits:
- A reduction in time and cost through automation (24/96 well formats).
- Improvement in product quality with measurements of previously undetectable changes in biomolecule structure.
- De-risking of downstream product failures through earlier up-stream monitoring.
- Generation of quality data for confident decision-making in order to accelerate development timelines.
The Apollo and AQS³pro directly measure protein secondary structure, making each a powerful tool for monitoring and understanding the mechanisms of protein stability and aggregation.
Protein quantitation is critically important in biopharmaceutical research and development labs in applications ranging from enzymatic studies to providing data for biopharmaceutical lot release.
Microfluidic Modulation Spectroscopy provides highly precise measurements of key secondary structure features, allowing new insights into even the smallest changes difficult to detect with existing technologies.
The Apollo and AQS³pro directly measure protein secondary structure, making it a powerful tool for monitoring and understanding the mechanisms of protein stability and aggregation.
Determining protein similarity for biologics is more challenging, compared to small molecules, due to their larger size and structural complexity. With the AQS³pro users can differentiate even the smallest of differences in structure and therefore confirm structural similarity with a high degree of precision.