Higher Quality Data, More Confident Decision-Making

RedShiftBio technologies deliver highly accurate results with the sensitivity, automation, and speed not possible with conventional techniques. This means you’ll have a clearer view of what’s happening with your process and your biomolecule exactly when you need it. Remove the delays, minimize the guesswork, say goodbye to bottlenecks. High-quality and high-resolution data to inform your decisions are now at your fingertips.

See Structural Changes with Greater Sensitivity

Microfluidic Modulation Spectroscopy

Protein higher order structure is a critical quality attribute that must be assessed as part of the drug development process and is essential to understanding the function of a given biomolecule.  The ability to assess protein structure, stability, and aggregation under biologically/ pharmacologically relevant concentrations and buffer/formulation conditions yields much more relevant information about the proteins, antibodies, peptides, and other biomolecules being studied. Discovering your protein or biologic drug is unstable or prone to aggregation too late in development is a very costly and avoidable scenario.

Microfluidic Modulation Spectroscopy (MMS) enables direct, label-free analysis of the secondary structure of proteins over a wide concentration range (0.1 – 200 mg/mL) in complex formulation buffers without the need for buffer exchange or dilution. With MMS, you’ll see changes in biomolecule attributes that are missed by other technologies while gaining critical insights across all phases of drug development and manufacturing.

Although second derivative spectra can be compared without area normalization, both FTIR and MMS replicate spectra showed improvement in linearity across a spiked BSA range after normalization, with MMS spectra already superior in R2 prior to processing. Figure 1 shows the effect of area normalization on second derivative FTIR spectra and Figure 1 shows the effect on spectra collected using MMS.
Both sets of replicate data covered the entire BSA impurity spike range. The FTIR data demonstrated a slight improvement in R2 from 0.4508 to 0.772 before and after area normalization by WSD with obvious lower reproducibility shown in the overlaid trace. This improvement is still significantly lower than the linearity observed using MMS, which already demonstrated a high R2 value of 0.9965 and greater reproducibility pre-area normalization, improving slightly to 0.9997 after area normalization.
Measure Protein Titer in Real-time

Purpose-built Liquid Chromatography

Accurate and reliable measurement of antibody concentration during process development and manufacturing is essential. While HPLC sets the standard for accuracy and dynamic range, the turnaround for results can be 24 hours or more, creating costly hold times and bottlenecks, and eliminating the ability to make real-time decisions.

In contrast,  the simple and purpose-built liquid chromatography solution provided by HaLCon delivers on-demand antibody titer measurements over a wide dynamic range of 0.1 – 10 g/L in just minutes with an accuracy that strongly correlates with off-line HPLC. This speed and accuracy supports real-time decision-making and continuous bioprocessing.

See how purpose-built protein A affinity chromatography compares to HPLC.

Simulated Cell Culture Titer

HaLCon vs HPLC Titer Correlation

Be confident in your titer measurements with HaLCon, which delivers protein titer concentration that correlates to off-line HPLC measurements across an extraordinary dynamic range.

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RedShiftBio AQS3 Product Detail