HaLCon is designed to integrate directly into your bioprocessing suite to provide real-time protein titer data without disrupting existing workflows or equipment.
When used with an automated sampling system, measurements can be obtained with a few button clicks to deliver actionable data-points so you don't have to wait for critical results.
Rapid IgG Titer Measurement
HaLCon, from RedShiftBio, is a cutting-edge automated bioreactor sampling solution that is purpose-built to provide real-time protein concentration measurements. HaLCon features a sample-to-answer time of less than five minutes, allowing you to accelerate upstream process development and downstream bioprocessing.
HaLCon Correlates with Off-Line HPLC Measurement
HaLCon’s affinity chromatography capabilities allow you to monitor titer by producing antibody concentration measurements that correlate to off-line HPLC, across an extraordinary dynamic range so you can be confident in your measurements.
Chromatography Without Complexity
HaLCon specifically employs protein A affinity chromatography and delivers IgG titer measurements without the wait time, method development, or specialized training required with HPLC. The whole system can be up and running in minutes so users can access a user-friendly interface to quickly achieve titer measurements with minimal effort. Through the addition of an autosampler, you can further improve your workflow by taking your bioprocess online to experience hands-free batch sampling. In addition, the sample flow path is completely contained in a consumable module to eliminate frequent re-validation.
Purpose-Built Liquid Chromatography System
HaLCon is designed to integrate directly into to provide real-time data without disrupting existing workflows and is designed specifically for protein concentration measurement in bioprocessing environments. HaLCon is purpose-built to quantify the amount of IgG in a cell-free sample, so you’ll benefit from a system optimized for efficient and accurate automated bioreactor sampling, to ensure reliable and consistent results with every use.
Real-time Data is Actionable Data
Access to fast, accurate, real-time titer data can ease your transition from upstream to downstream by minimizing uncertainty, costly hold times, and bottlenecks. When used with an automated sampling system, measurements can be obtained with a few button clicks to deliver actionable data points allowing you to make critical decisions in real-time, so you can respond promptly to changes in titer levels for efficient bioprocessing.
Compact Space-Saving Design
HaLCon’s small footprint and minimal connections make it easy to integrate into your bioprocess development workflow.
How much sample material or sample volume is required to run HaLCon?
We recommend 100 µL per sample. If you have a lower desired sample volume, please contact us and one of our experts can help evaluate the feasibility of the proposed sample volume.
How does HaLCon react to other Immunoglobulins and other impurities?
The Tridex uses a Protein A media. Anything not bound to the Protein A passes through the column to waste.
What is the HaLCon "trap-and-elute technique" and how does it work?
Trap and elute means the analyte (IgG) is fully captured on the column, everything else passes through, then the analyte is released and the detector sees one peak. In terms of flow it differs from traditional liquid chromatography in that there is no gradient, just 100% reagent A to capture or trap IgG, then 100% reagent B to elute.
What type of maintenance is required for HaLCon? And, will this maintenance be performed during a yearly PM?
No regular maintenance is required beyond changing the reagent pack and analysis module every 3 months or after running 1,000 samples, whichever comes first. It is recommended to run a flush before running samples if the system has been idle for a few days.