HaLCon: Real-Time Protein Titer Measurement for Bioprocessing
Accurate, Actionable Protein Titer in Minutes, Not Days
Introducing HaLCon from RedShiftBio - the fastest, simplest affinity chromatography solution for real-time protein concentration determination, at-line or on-line, for bioprocess development and bioprocess manufacturing.
HaLCon Key Features
- Rapid Titer Measurement - Under 5 Minutes
- Correlation with Off-Line HPLC Measurement
- Contained Sample Flow Path
- Ease of Use
- Purpose-Built Protein Liquid Chromatography System
- Enhanced Monitoring Capability
- Compact Space-Saving Design
Want to learn how you can use real-time antibody titer measurements to extract the most value from every bioreactor run?
Connect with our team to learn more about HaLCon and how it can benefit your bioprocess development.
HaLCon: Real-Time Protein Titer Measurement for Bioprocessing
Accurate, Actionable Protein Titer in Minutes, Not Days
Accurate, Actionable Protein Titer in Minutes, Not Days
Introducing HaLCon from RedShiftBio - the fastest, simplest affinity chromatography solution for real-time protein concentration determination, at-line or on-line, for bioprocess development and bioprocess manufacturing.
HaLCon Key Features
- Rapid Titer Measurement - Under 5 Minutes
- Correlation with Off-Line HPLC Measurement
- Contained Sample Flow Path
- Ease of Use
- Purpose-Built Protein Liquid Chromatography System
- Enhanced Monitoring Capability
- Compact Space-Saving Design
HaLCon is the fastest, simplest solution for protein titer measurements.
HaLCon is designed to integrate directly into your bioprocessing suite to provide real-time protein titer data without disrupting existing workflows or equipment.
When used with an automated sampling system, measurements can be obtained with a few button clicks to deliver actionable data-points so you don't have to wait for critical results.
Why Choose HaLCon from RedShiftBio?
Rapid IgG Titer Measurement
HaLCon, from RedShiftBio, is a cutting-edge automated bioreactor sampling solution that is purpose-built to provide real-time protein concentration measurements. HaLCon features a sample-to-answer time of less than five minutes, allowing you to accelerate upstream process development and downstream bioprocessing.
HaLCon Correlates with Off-Line HPLC Measurement
HaLCon’s affinity chromatography capabilities allow you to monitor titer by producing antibody concentration measurements that correlate to off-line HPLC, across an extraordinary dynamic range so you can be confident in your measurements.
Chromatography Without Complexity
HaLCon specifically employs protein A affinity chromatography and delivers IgG titer measurements without the wait time, method development, or specialized training required with HPLC. The whole system can be up and running in minutes so users can access a user-friendly interface to quickly achieve titer measurements with minimal effort. Through the addition of an autosampler, you can further improve your workflow by taking your bioprocess online to experience hands-free batch sampling. In addition, the sample flow path is completely contained in a consumable module to eliminate frequent re-validation.
Purpose-Built Liquid Chromatography System
HaLCon is designed to integrate directly into to provide real-time data without disrupting existing workflows and is designed specifically for protein concentration measurement in bioprocessing environments. HaLCon is purpose-built to quantify the amount of IgG in a cell-free sample, so you’ll benefit from a system optimized for efficient and accurate automated bioreactor sampling, to ensure reliable and consistent results with every use.
Real-time Data is Actionable Data
Access to fast, accurate, real-time titer data can ease your transition from upstream to downstream by minimizing uncertainty, costly hold times, and bottlenecks. When used with an automated sampling system, measurements can be obtained with a few button clicks to deliver actionable data points allowing you to make critical decisions in real-time, so you can respond promptly to changes in titer levels for efficient bioprocessing.
Compact Space-Saving Design
HaLCon’s small footprint and minimal connections make it easy to integrate into your bioprocess development workflow.
Frequently Asked Questions
How much sample material or sample volume is required to run HaLCon?
We recommend 100 µL per sample. If you have a lower desired sample volume, please contact us and one of our experts can help evaluate the feasibility of the proposed sample volume.
How does HaLCon react to other Immunoglobulins and other impurities?
The Tridex uses a Protein A media. Anything not bound to the Protein A passes through the column to waste.
What is the HaLCon "trap-and-elute technique" and how does it work?
Trap and elute means the analyte (IgG) is fully captured on the column, everything else passes through, then the analyte is released and the detector sees one peak. In terms of flow it differs from traditional liquid chromatography in that there is no gradient, just 100% reagent A to capture or trap IgG, then 100% reagent B to elute.
What type of maintenance is required for HaLCon? And, will this maintenance be performed during a yearly PM?
No regular maintenance is required beyond changing the reagent pack and analysis module every 3 months or after running 1,000 samples, whichever comes first. It is recommended to run a flush before running samples if the system has been idle for a few days.
