Protein Titer in Minutes.
Without Waiting for HPLC.
HaLCon delivers HPLC-level accuracy in 5 minutes, using only 100 µL of sample.
HaLCon is our proprietary liquid chromatography system engineered to deliver rapid, accurate protein analysis without complex method development. Designed for continuous bioprocess manufacturing, HaLCon replaces slow offline HPLC workflows with real-time protein titer measurement.
From early cell growth to high-yield harvests,
HaLCon tracks IgG protein titers in real time.
Measure protein titers from ~0.1 g/L to 8 g/L with confidence, knowing HaLCon delivers results that match offline HPLC (R² > 0.99).
Simulated Cell Culture Titer
HaLCon vs HPLC Titer Correlation
Where HaLCon Fits in Your Workflow
DoE Cycles: Run more experiments faster by eliminating HPLC bottlenecks.
Harvest Timing: Optimize monoclonal antibody production with titer results in minutes, not hours.
Perfusion Monitoring: Spot column breakthrough in real time to prevent wasted product.
Column Loading: Load Protein A columns with precision and extend resin life.
HaLCon vs Other Protein Titer Methods
| Method | Time to Result | Accuracy | Reproducibility | Sample Volume | Ease of Use | Throughput |
|---|---|---|---|---|---|---|
HaLCon | 5 min | HPLC-level (R² > 0.99) | High (>98%) | 100 µL | Fully automated, minimal training | High (Nest autosampler) |
HPLC (Protein A) | 10–60min per sample (plus que time) | Gold standard | High | 500+ µL | Skilled operator, complex method development | Low–Medium |
ELISA/ Immunoassay | Hours–days | High specificity | Variable (operator/lab dependent) | 50–200 µL | Moderate training, manual steps | Low |
Biosensors (Cedex® Bio HT, Octet) | 15–30 min | Moderate (matrix-dependent) | Moderate | 50–100 µL | Moderate; training required | Medium |
Spectroscopy (Ultraviolet, absorbance, scattering) | Seconds–minutes | Low | Low | 50–200 µL | Easy | Low |
