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Behind the App Note: Detection of Pressure-Induced Protein Aggregation Using Microfluidic Modulation Spectroscopy (MMS)

December 6, 2022

The ability to detect protein aggregation is important at all stages of drug development. Early detection of protein aggregation is most desirable to inform development decisions, as it is a recognized signal of instability and can lead to the loss of protein function. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents, was employed to create aggregated human gamma-globulin for this spiked study.‍

Dr. Valerie Collins, Applications Manager at RedShiftBio hosts this video to highlight how ultra-sensitive MMS was used to obtain aggregation measurements that were previously undetectable by other methods and can greatly influence the direction of biopharma product development: 

  • ​​​​​​Significant changes present in the spectral regions of 1624 and 1640cm-1 in the Second Derivative plot that can be seen only subtly in the Absolute Absorbance spectral plot
  • The use of Weighted Spectral Difference (WSD) as a metric to monitor change in sample secondary structure 
  • The strong linear correlation between increasing amounts of percent aggregation and increasing amounts of measured antiparallel beta-sheet content

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