The ability to detect protein aggregation is important at all stages of drug development. Early detection of protein aggregation is most desirable to inform development decisions, as it is a recognized signal of instability and can lead to the loss of protein function. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents, was employed to create aggregated human gamma-globulin for this spiked study.
Dr. Valerie Collins, Applications Manager at RedShiftBio hosts this video to highlight how ultra-sensitive MMS was used to obtain aggregation measurements that were previously undetectable by other methods and can greatly influence the direction of biopharma product development:
- Significant changes present in the spectral regions of 1624 and 1640cm-1 in the Second Derivative plot that can be seen only subtly in the Absolute Absorbance spectral plot
- The use of Weighted Spectral Difference (WSD) as a metric to monitor change in sample secondary structure
- The strong linear correlation between increasing amounts of percent aggregation and increasing amounts of measured antiparallel beta-sheet content