HaLCon Protein Analyzer: A Study on At-Line Titer Analysis in Biopharmaceutical Applications

This video introduces the HALCON Protein Analyzer and its integration into clinical manufacturing workflows for real-time titer measurement. Designed for speed and ease of use, the HALCON system uses a protein-A affinity column for accurate, in-process titer analysis of therapeutic proteins. The system was evaluated across four different BMS molecules, showing strong correlation with UPLC reference methods in terms of precision and bias. Despite minor challenges like injection loop cleaning and carryover risk, HALCON significantly reduces turnaround time while requiring minimal operator training—making it a practical solution for modern bioprocess environments.