Thermal Denaturation Analysis of Bovine Serum Albumin over Wide Concentration Range by Microfluidic Modulation Spectroscopy

APPLICATION NOTE

Thermal Denaturation Analysis of Bovine Serum Albumin over Wide Concentration Range by Microfluidic Modulation Spectroscopy

This Application Note Includes:

• In this App, MMS was used to study the heat-induced thermal denaturation of BSA samples
• Allows the use of simpler detectors without the need for liquid nitrogen cooling
• Differentiates small changes in secondary structure across a wide concentration range without the interference of excipients or loss of linearity at the lowest and highest concentrations
• Delivers information about aggregation, stability, HOS, and similarity in addition to quantitation results

Secondary Structure Analysis Tools For Measuring Thermal Denaturation

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1). MMS is performed on the AQS3pro system from RedShift BioAnalytics, and it generates measurements over a wide range of complex backgrounds, including buffers that contain absorbing materials and scattering components such as reducing agents, adjuvants, surfactants, and other excipients.