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App Notes

App Notes

Secondary Structure Determination using Microfluidic Modulation Spectroscopy in the Presence of DMSO

October 1, 2020

APPLICATION NOTE

This Application Note Includes:

  • lysozyme was dissolved in a buffer containing 0, 5, and 10% DMSO
  • MMS generates an output that is 100 times brighter than traditional FTIR light sources  by utilizing the AQS³pro's Quantum Cascade Laser as the IR light source
  • MMS can successfully be used for secondary structure determination in the presence of DMSO
  • MMS results in a much greater signal-to-noise ratio and high sensitivity for accurate measurement of proteins ranging from 0.1 to over 200 mg/mL

Secondary Structure Determination In The Presence Of DMSO

Characterizing biologic drug products is crucial in drug development and the presence of organic solvents and optically active excipients typically hinders traditional characterization techniques. RedShiftBio has developed the AQS³pro, a novel characterization system powered by Microfluidic Modulation Spectroscopy (MMS) as a solution to overcoming these obstacles. MMS combines Infrared (IR) spectroscopy with automatic sample handling and a micro-flow cell for accurate measurements with no buffer interference. The system uses rapid modulation between a sample and its reference buffer to generate a differential spectrum for the most accurate buffer subtraction.  This app note demonstrates that MMS can be used as an alternative to traditional secondary structure determination tools, when organic solvents and optically active excipients are present.

Due to the rapid modulation between sample and buffer, MMS successfully subtracts background to better structurally charcacterize proteins.

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