Microfluidic Modulation Spectroscopy: Monitoring Protein Structure During ReFolding & Thermal Stress

‍David Sloan, PhD

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Microfluidic Modulation Spectroscopy (MMS) for Monitoring Protein Structure During Re-Folding and Thermal StressProtein stability is a critical quality attribute for a new biologic drug. To investigate stability through folding and unfolding, we stressed multiple proteins both thermally and with pressure and measured the changes in the protein structure and melting point (Tm) using MMS. We will also discuss a novel method for re-solubilizing and re-folding proteins using urea and pressure resulting in natively folded proteins whose structure matches that of the original unstressed protein.