RedShiftBio Lab Photo

App Notes

App Notes

Detection of Pressure-Induced Protein Aggregation Using Microfluidic Modulation Spectroscopy (MMS)

April 8, 2021


The ability to detect protein aggregation is important at all stages of drug development. Early detection of aggregation is most desirable to inform development decisions since aggregation can negatively affect the functionality of a protein.

This Application Note Includes:

  • Highlights the significant changes present in the spectral regions of 1624 and 1640cm-1 in the Second Derivative plot that can be seen only subtly in the Absolute Absorbance spectral plot.
  • Weighted Spectral Difference (WSD) as a metric used to monitor change in sample secondary structure for this aggregation study
  • Strong linear correlation between increasing amounts of percent aggregation and increasing amounts of measured antiparallel beta-sheet content

Pressure-Induced Protein Aggregation Detection
Protein aggregation is a recognized signal of instability and can lead to the loss of protein function. It is therefore crucial to detect protein aggregation early in the drug development process to inform further drug development decisions. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents1, was employed to create aggregated protein for this spiked study.

The ability to see change in antiparallel beta sheet composition through stability monitoring by MMS creates an advantage of correlating drug development conditions to potential detrimental affects on protein function at all stages of development.

Please complete the form if you would like to download the app note.

Thank you for submitting the form. A member of our team will reach out shortly.
Oops! Something went wrong while submitting the form.

Request a Demo Today! 

RedShiftBio AQS3 Product Detail